Evaluation of the effect of ellagic acid on muscle differentiation
Farah, Sonia Jihad
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This thesis investigates the potential impact of ellagic acid (EA), a polyphenol present in various fruits and nuts, on in vitro muscle differentiation using the C2C12 mouse myoblast cell line. Muscle differentiation is a complex process involving specific genes and signaling pathways, and recent interest in natural compounds, including EA, has sparked exploration into their effects on this critical physiological process. The study aims to determine effective and non-cytotoxic concentrations of EA, analyze myotube formation, and evaluate the expression of key differentiation markers at both gene and protein levels. The findings reveal the safety and efficacy of EA at specific concentrations. Morphological, as well as gene and protein expression analyses provide insights into the initiation and progression of myoblast differentiation, emphasizing EA's positive impact on essential markers. This research not only confirms the initial hypothesis but also positions EA as a potent promoter of muscle differentiation, offering potential therapeutic applications for muscle regeneration and disorders. Future directions include exploring EA in pathological muscle conditions and addressing challenges in oral bioavailability through innovative delivery strategies. The ongoing journey into the unknown seeks to deepen our understanding of EA and its broader implications for muscle health.