dc.creator | Richard Willson | |
dc.date | 2015 | |
dc.date.accessioned | 2018-10-18T21:22:00Z | |
dc.date.available | 2018-10-18T21:22:00Z | |
dc.identifier.issn | 19326203 | |
dc.identifier.doi | 10.1371/journal.pone.0126571 | |
dc.identifier.uri | http://hdl.handle.net/11285/630453 | |
dc.description | Noroviruses are recognized worldwide as the principal cause of acute, non-bacterial gastroenteritis, resulting in 19-21 million cases of disease every year in the United States. Noroviruses have a very low infectious dose, a short incubation period, high resistance to traditional disinfection techniques and multiple modes of transmission, making early, point-of-care detection essential for controlling the spread of the disease. The traditional diagnostic tools, electron microscopy, RT-PCR and ELISA require sophisticated and expensive instrumentation, and are considered too laborious and slow to be useful during severe outbreaks. In this paper we describe the development of a new, rapid and sensitive lateral-flow assay using labeled phage particles for the detection of the prototypical norovirus GI.1 (Norwalk), with a limit of detection of 107 virus-like particles per mL, one hundredfold lower than a conventional gold nanoparticle lateral-flow assay using the same antibody pair. © 2015 Hagström et al. | |
dc.language | eng | |
dc.publisher | Public Library of Science | |
dc.relation | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84929377736&doi=10.1371%2fjournal.pone.0126571&partnerID=40&md5=615a77665329f810f1045846a97e6e70 | |
dc.relation | Investigadores | |
dc.relation | Estudiantes | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0 | |
dc.source | PLoS ONE | |
dc.subject | gold nanoparticle | |
dc.subject | nanoparticle | |
dc.subject | phage nanoparticle reporter | |
dc.subject | unclassified drug | |
dc.subject | nanoparticle | |
dc.subject | antigen binding | |
dc.subject | Article | |
dc.subject | assay | |
dc.subject | controlled study | |
dc.subject | diagnostic test accuracy study | |
dc.subject | enzyme linked immunosorbent assay | |
dc.subject | intermethod comparison | |
dc.subject | lateral flow assay | |
dc.subject | limit of detection | |
dc.subject | nonhuman | |
dc.subject | Norwalk virus | |
dc.subject | sensitivity and specificity | |
dc.subject | virus detection | |
dc.subject | virus like agent | |
dc.subject | bacteriophage | |
dc.subject | bioassay | |
dc.subject | Caliciviridae Infections | |
dc.subject | Escherichia coli | |
dc.subject | gastroenteritis | |
dc.subject | genetics | |
dc.subject | human | |
dc.subject | isolation and purification | |
dc.subject | metabolism | |
dc.subject | Norovirus | |
dc.subject | procedures | |
dc.subject | United States | |
dc.subject | virology | |
dc.subject | Bacteria (microorganisms) | |
dc.subject | Norovirus | |
dc.subject | Bacteriophages | |
dc.subject | Biological Assay | |
dc.subject | Caliciviridae Infections | |
dc.subject | Escherichia coli | |
dc.subject | Gastroenteritis | |
dc.subject | Humans | |
dc.subject | Nanoparticles | |
dc.subject | Norovirus | |
dc.subject | Sensitivity and Specificity | |
dc.subject | United States | |
dc.subject.classification | 7 INGENIERÍA Y TECNOLOGÍA | |
dc.title | Sensitive detection of norovirus using phage nanoparticle reporters in lateral-flow assay | |
dc.type | Artículo | |
dc.identifier.volume | 10 | |
dc.identifier.issue | 5 | |
refterms.dateFOA | 2018-10-18T21:22:00Z | |