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dc.contributor.advisorGutiérrez Uribe, Janet Alejandra
dc.contributor.authorGarcía Campuzano, Jacqueline
dc.identifier.citationGarcía Campuzano, J.(2022). Coffee pulp polysaccharides with potential immunomodulatory activity [Unpublished master's thesis]. Instituto Tecnológico y de Estudios Superiores de Monterrey.es_MX
dc.description.abstractCoffee is one of the most valuable primary products in world trade and its agricultural production and consumption are part of Mexican culture. Coffee pulp is the first byproduct obtained during the wet processing of coffee. This waste is rich in macronutrients and contains considerable amounts of tannins, total pectic substances, sugars, caffeine, chlorogenic acid and caffeic acid. These components have multiple beneficial health properties. This has led to the generation of a line of research for the study of biologically active ingredients such as specific polysaccharides that may present immunostimulatory activity for the treatment of diseases. The following project assessed the immunomodulatory potential of different pectin extracts from coffee pulp. The extraction of polysaccharides that may have an immunomodulatory activity was the main objective of this investigation along with the identification and quantification of phenolic compounds present in the extracts. The interactions between the polysaccharides and phenolic compounds present in the extracts are key for understanding their dual immunomodulatory effect. This could contribute to the treatment and prevention of immunodeficiency diseases. This project was structured in three main stages: extraction of pectin from coffee pulp, separation and quantification of phenolic compounds in the extracts and evaluation of the immunomodulatory potential of the polysaccharides. Dry coffee pulp samples were collected from Tezonapa, Veracruz. Pectin extractions were carried out with different experimental factors, such as the type of solvent (water and acidified water to pH 4 using citric acid at a 1% concentration), pulp: solvent ratio (1:10 and 1:20), agitation (with and without agitation), temperature (90 °C and 70 °C) and washing method (dialysis and ethanol washes). The content of total phenolic compounds, caffeine and caffeic acid of the extracted pectin samples was also evaluated. Finally, cytotoxicity assays in mouse machrophages cell line (RAW264.7) were performed to evaluate the growth induction of the different extracts. LPSinduced inflammatory response of this cell line was also evaluated.es_MX
dc.publisherInstituto Tecnológico y de Estudios Superiores de Monterreyes_MX
dc.titleCoffee pulp polysaccharides with potential immunomodulatory activityes_MX
dc.typeTesis de Maestría / master Thesises_MX
dc.contributor.departmentSchool of Engineering and Scienceses_MX
dc.rights.embargoreasonPeriodo predeterminado para revisión de contenido susceptible de protección, patente o comercialización.es_MX
dc.contributor.committeememberLuna Vital, Diego Armando
dc.contributor.committeememberAguilar Jiménez, Oscar Alejandro
dc.identifier.orcid /0000-0003-3620-1835es_MX
dc.subject.keywordCoffee pulpes_MX
dc.subject.keywordPhenolic compoundses_MX
dc.contributor.institutionCampus Monterreyes_MX
dc.description.degreeMaster of Science In Biotechnologyes_MX
dc.audience.educationlevelPúblico en general/General publices_MX

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