Caracterización y rendimiento de células madre mesenquimales CD133+ y CD133- derivadas de tejido adiposo
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Abstract
Adipose-derived mesenchymal stem cells are adult multipotent cells that have shown
promising results in regenerative medicine. Heterogeneity among this cell group poses a
challenge for clinical application. The following research focuses on characterizing and
quantifying the yield of a subpopulation expressing the pluripotency marker, CD133
(Prominin1), which identifies early progenitor cells. Adipose-derived mesenchymal stem cells
were isolated from lipoaspirate tissue of five healthy female subjects. CD133+ and CD133-
stem cells were obtained through magnetic-activated cell sorting. Around 1% of mononuclear
cells were CD133+ after isolation. Both fractions meet the minimal criteria for mesenchymal
stem cells of the International Society of Cellular Therapy. End-point RT-PCR and flow
cytometry analysis reveal significant differences between CD133+ and CD133- fractions in
terms of pluripotency markers. Neither the positive nor the negative fractions were positive for
CD133 at mRNA or protein levels in our culture conditions. Therefore, we conclude that
CD133 yield in ADSC is low and the membrane protein is lost after culturing cells with
standard culture media.