Caracterización y rendimiento de células madre mesenquimales CD133+ y CD133- derivadas de tejido adiposo
Giacoman Lozano, Mayela
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Adipose-derived mesenchymal stem cells are adult multipotent cells that have shown promising results in regenerative medicine. Heterogeneity among this cell group poses a challenge for clinical application. The following research focuses on characterizing and quantifying the yield of a subpopulation expressing the pluripotency marker, CD133 (Prominin1), which identifies early progenitor cells. Adipose-derived mesenchymal stem cells were isolated from lipoaspirate tissue of five healthy female subjects. CD133+ and CD133- stem cells were obtained through magnetic-activated cell sorting. Around 1% of mononuclear cells were CD133+ after isolation. Both fractions meet the minimal criteria for mesenchymal stem cells of the International Society of Cellular Therapy. End-point RT-PCR and flow cytometry analysis reveal significant differences between CD133+ and CD133- fractions in terms of pluripotency markers. Neither the positive nor the negative fractions were positive for CD133 at mRNA or protein levels in our culture conditions. Therefore, we conclude that CD133 yield in ADSC is low and the membrane protein is lost after culturing cells with standard culture media.