dc.contributor.advisor | González Garza y Barrón, María Teresa | |
dc.contributor.advisor | Adachi, Roberto | |
dc.creator | González Delgado, Ricardo | |
dc.date.accessioned | 2019-01-03T15:07:02Z | |
dc.date.available | 2019-01-03T15:07:02Z | |
dc.date.created | 2018 | |
dc.identifier.citation | Gonzalez, R. (2018). Syntaxin 11 regulates platelet granule release and hemostasis. N.A., a, 100. | en_US |
dc.identifier.uri | http://hdl.handle.net/11285/632723 | |
dc.description.abstract | Degranulation, a form of regulated exocytosis, is essential for platelet participation in hemostasis.
The interaction between Syntaxin (Stx) and Munc18 proteins is required for exocytosis. We have
proven that selective genetic deletion of Munc18-2 eliminates regulated exocytosis in platelets,
but its Stx partner was not known. Among the exocytic Stx (1A, 1B, 2, 3, 4, and 11), we proved
that Stxs1A, 1B, and 3 are not expressed platelets. Others have shown that deletion of Stx2 and
4 had no consequences on platelet degranulation. Now our goal is to study if Stx11 is involved in
platelet exocytosis. We created a Stx11 conditional knockout mouse and crossed it with a
platelet-specific Cre mouse to generate cell-specific Stx11 deletants. We evaluated the exocytosis
of individual subpopulations of platelet granules stimulated with thrombin or collagen. Platelets
lacking Stx11 had a marked decrease in dense granule exocytosis with both agonists, but alpha
and lysosomal granules were only affected when thrombin was used. Stx11-deficient platelets
had no deficiency in granule biogenesis and were capable of undergoing full activation. Platelets
from Stx11 deletant mice had deficient aggregation and ex vivo thrombus formation. In vivo, all
these defects translated into protection from arterial thrombosis, and in abnormal hemostasis after
venous and arterial injury, in mice lacking Stx11 in their platelets. | en_US |
dc.format.extent | 100 | en_US |
dc.format.medium | Texto | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Instituto Tecnológico y de Estudios Superiores de Monterrey | esp |
dc.relation.ispartof | 266632-CONACYT-SENER-S0019201401 | en_US |
dc.rights | Open Access | en_US |
dc.subject | 3 MEDICINA Y CIENCIAS DE LA SALUD | en_US |
dc.title | Syntaxin 11 regulates platelet granule release and hemostasis | en_US |
dc.type | Tesis de Maestría / Master Thesis | en_US |
dc.contributor.mentor | Aguilar, Oscar | |
dc.publisher.institution | Instituto Tecnológico y de Estudios Superiores de Monterrey | en_US |
dc.subject.keyword | Syntaxin 11 | en_US |
dc.subject.keyword | SNARE complex | en_US |
dc.subject.keyword | Platelet | en_US |
dc.subject.keyword | Regulated exocytosis | en_US |
dc.subject.keyword | Granules | en_US |
dc.subject.keyword | Hemostasis | en_US |
dc.contributor.institution | Campus Monterrey | en_US |
dc.contributor.institution | Campus Monterrey | en_US |
dc.contributor.institution | Campus Monterrey | en_US |
dc.subject.discipline | Ciencias de la Salud / Health Sciences | en_US |
dc.description.degree | Master of Science in Biotehcnology | en_US |
dc.audience.educationlevel | Estudiantes/Students | en_US |
dc.relation.impreso | 2018-11-26 | |