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Human adipose derived stem cells regress fibrosis in a chronic renal fibrotic model induced by adenine

dc.creatorJuan Socorro Armendariz Borunda
dc.date2017
dc.date.accessioned2018-10-18T22:08:30Z
dc.date.available2018-10-18T22:08:30Z
dc.identifier.issn19326203
dc.identifier.doi10.1371/journal.pone.0187907
dc.identifier.urihttp://hdl.handle.net/11285/630599
dc.descriptionBackground and aims: ADSCs transplantation had been shown in some experimental models of kidney damage that it improves kidney function and reduces fibrosis. In this study we evaluated the effect of human adipose tissue-derived stem cell (hADSC) therapy in a chronic kidney damage experimental model. Methods A chronic kidney injury was induced by daily orogastric administration of adenine (100mg/ kg) to male Wistar rats for 28 days. hADSCs were isolated, expanded and characterized before transplantation. hADSC administration was performed in a tail vein at a dose of 2 x106 cells/animal. Animals were sacrificed at 7 days post-treatment. The percentage of fibrotic tissue, serum and urine levels of urea, creatinine, total protein and renal mRNA of COL1A1, TGFB1, CTGF, ACTA2, IL6, IL10, TNF were analyzed. Results hADSCs treatment significantly reduces kidney fibrosis, improves urea and creatinine serum and urine levels, and diminishes COL1A1, TGFB1, CTGF, ACTA2 mRNA kidney levels. Conclusions These results showed that cell therapy using hADSCs improves renal function and reduces fibrosis. © 2017 Rivera-Valdés et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.languageeng
dc.publisherPublic Library of Science
dc.relationhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85039743902&doi=10.1371%2fjournal.pone.0187907&partnerID=40&md5=1f5d5b16c052b3fc4136407bb4298def
dc.relationInvestigadores
dc.relationEstudiantes
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.sourcePLoS ONE
dc.subjectACTA2 protein
dc.subjectadenine
dc.subjectalpha smooth muscle actin
dc.subjectCOL1A1 protein
dc.subjectcollagen
dc.subjectconnective tissue growth factor
dc.subjectcreatinine
dc.subjectmessenger RNA
dc.subjecttransforming growth factor beta1
dc.subjectunclassified drug
dc.subjecturea
dc.subjectadenine
dc.subjectadipose derived stem cell
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectArticle
dc.subjectcontrolled study
dc.subjectcreatinine blood level
dc.subjectcreatinine urine level
dc.subjecthuman
dc.subjecthuman cell
dc.subjectkidney fibrosis
dc.subjectmale
dc.subjectnonhuman
dc.subjectrat
dc.subjectstem cell transplantation
dc.subjecttreatment outcome
dc.subjecturea blood level
dc.subjectadipose tissue
dc.subjectanimal
dc.subjectcell culture
dc.subjectchemically induced
dc.subjectcytology
dc.subjectfibrosis
dc.subjectgene expression profiling
dc.subjectgenetics
dc.subjectkidney disease
dc.subjectstem cell
dc.subjectWistar rat
dc.subjectAdenine
dc.subjectAdipose Tissue
dc.subjectAnimals
dc.subjectCells, Cultured
dc.subjectFibrosis
dc.subjectGene Expression Profiling
dc.subjectHumans
dc.subjectKidney Diseases
dc.subjectMale
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectStem Cells
dc.subject.classification7 INGENIERÍA Y TECNOLOGÍA
dc.titleHuman adipose derived stem cells regress fibrosis in a chronic renal fibrotic model induced by adenine
dc.typeArtículo
dc.identifier.volume12
dc.identifier.issue12
refterms.dateFOA2018-10-18T22:08:30Z


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