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Identification and Characterization of microRNAS from Entamoeba histolytica HM1-IMSS

dc.creatorVíctor Manuel Treviño Alvarado
dc.date2013
dc.date.accessioned2018-10-18T21:51:09Z
dc.date.available2018-10-18T21:51:09Z
dc.identifier.issn19326203
dc.identifier.doi10.1371/journal.pone.0068202
dc.identifier.urihttp://hdl.handle.net/11285/630518
dc.descriptionBackground:Entamoeba histolytica is the causative agent of amebiasis, a disease that is a major source of morbidity and mortality in the developing world. MicroRNAs (miRNAs) are a large group of non-coding RNAs that play important roles in regulating gene expression and protein translation in animals. Genome-wide identification of miRNAs is a critical step to facilitating our understanding of genome organization, genome biology, evolution, and post-transcriptional regulation.Methodology/Principal Findings:We sequenced a small RNA library prepared from a culture of trophozoites of Entamoeba histolytica Strain HM1-IMSS using a deep DNA sequencing approach. Deep sequencing yielded 16 million high-quality short sequence reads containing a total of 5 million non-redundant sequence reads. Based on a bioinformatics pipeline, we found that only 0.5% of these non-redundant small RNA reads were a perfect match with the drafted E. histolytica genome. We did not find miRNA homologs in plant or animal miRNAs. We discovered 199 new potential Entamoeba histolytica miRNAs. The expression and sequence of these Ehi-miRNAs were further validated through microarray by μParaflo Microfluidic Biochip Technology. Ten potential miRNAs were additionally confirmed by real time RT-PCR analysis. Prediction of target genes matched 32 known genes and 34 hypothetical genes.Conclusions/Significance:These results show that there is a number of regulatory miRNAs in Entamoeba histolytica. The collection of miRNAs in this parasite could be used as a new platform to study genomic structure, gene regulation and networks, development, and host-parasite interactions. © 2013 Mar-Aguilar et al.
dc.languageeng
dc.relationhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84880066426&doi=10.1371%2fjournal.pone.0068202&partnerID=40&md5=3fce81b5747895fc8e365750d94e2795
dc.relationInvestigadores
dc.relationEstudiantes
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.sourcePLoS ONE
dc.subjectmicroRNA
dc.subjectmicroRNA 12
dc.subjectmicroRNA 13
dc.subjectmicroRNA 144
dc.subjectmicroRNA 2
dc.subjectmicroRNA 24
dc.subjectmicroRNA 29
dc.subjectmicroRNA 46
dc.subjectmicroRNA 47
dc.subjectmicroRNA 5
dc.subjectmicroRNA 8
dc.subjectprotozoal RNA
dc.subjectunclassified drug
dc.subjectarticle
dc.subjectbiochip
dc.subjectbioinformatics
dc.subjectcontrolled study
dc.subjectDNA sequence
dc.subjectEntamoeba histolytica
dc.subjectgene expression
dc.subjectgene library
dc.subjectgenome analysis
dc.subjectmicroarray analysis
dc.subjectmicrofluidic analysis
dc.subjectnonhuman
dc.subjectreal time polymerase chain reaction
dc.subjectRNA analysis
dc.subjectsequence analysis
dc.subjectsequence homology
dc.subjecttrophozoite
dc.subjectCluster Analysis
dc.subjectDatabases, Genetic
dc.subjectEntamoeba histolytica
dc.subjectGenes, Protozoan
dc.subjectMicroRNAs
dc.subjectOligonucleotide Array Sequence Analysis
dc.subjectReal-Time Polymerase Chain Reaction
dc.subjectReproducibility of Results
dc.subjectSequence Analysis, RNA
dc.subjectTrophozoites
dc.subject.classification7 INGENIERÍA Y TECNOLOGÍA
dc.titleIdentification and Characterization of microRNAS from Entamoeba histolytica HM1-IMSS
dc.typeArtículo
dc.identifier.volume8
dc.identifier.issue7
refterms.dateFOA2018-10-18T21:51:09Z


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