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dc.creatorVíctor Arízaga Ballesteros
dc.creatorVíctor Javier Lara Díaz
dc.creatorJorge Eugenio Moreno Cuevas
dc.date2013
dc.date.accessioned2018-10-18T21:51:02Z
dc.date.available2018-10-18T21:51:02Z
dc.identifier.issn12019712
dc.identifier.doi10.1016/j.ijid.2012.09.005
dc.identifier.urihttp://hdl.handle.net/11285/630491
dc.descriptionObjectives: To study the potential of buffy coat culture as a diagnostic tool for neonatal late-onset sepsis. Methods: This was a study of diagnostic accuracy in newborn infants born at 28-41 weeks of gestation, weighing >800. g, with ≥8 points on the NOSEP-1 scale. Paired samples for total blood culture (TBC) and buffy coat culture were drawn. We established the positivity rate, sensitivity, specificity, predictive values, and likelihood ratios, and compared time to positivity and contamination rates. Results: Fifty-two newborns were included in the study. Twenty-one TBC and 22 buffy coat cultures were positive. The positivity rate for TBC was 40.4% and for buffy coat culture was 42.3% (p=. not significant). Three TBC were positive with negative buffy coat culture. Four buffy coat cultures were positive with negative TBC; Kappa agreement was 0.723, p <0.001. Buffy coat culture sensitivity was 86% (95% confidence interval (CI) 68.5-95.4%), specificity 87% (75.4-93.7%), positive predictive value 82% (65.4-91.1%), negative predictive value 90% (77.9-96.8%), positive likelihood ratio 6.64 (2.79-15.05), and negative likelihood ratio 0.16 (0.05-0.42). We found no difference in time to positivity in hours; Wilcoxon Z=. 1224, p=. 0.22. The contamination rate was 1.9% for both methods. Conclusions: Buffy coat culture is as good as TBC for the microbiological diagnosis of late-onset sepsis of the newborn. Buffy coat culture allows the use of remaining plasma for further analysis. © 2012 International Society for Infectious Diseases.
dc.languageeng
dc.relationhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84873474117&doi=10.1016%2fj.ijid.2012.09.005&partnerID=40&md5=2bb7740c3edab370344595d3c98b49c2
dc.relationInvestigadores
dc.relationEstudiantes
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.sourceInternational Journal of Infectious Diseases
dc.subjectarticle
dc.subjectblood culture
dc.subjectblood examination
dc.subjectbuffy coat culture
dc.subjectdiagnostic accuracy
dc.subjectfemale
dc.subjecthematological procedure
dc.subjecthuman
dc.subjectintermethod comparison
dc.subjectmajor clinical study
dc.subjectmale
dc.subjectnewborn
dc.subjectnewborn sepsis
dc.subjectpredictive value
dc.subjectprospective study
dc.subjectsensitivity and specificity
dc.subjecttotal blood culture
dc.subjectBacteriological Techniques
dc.subjectBlood Buffy Coat
dc.subjectEarly Diagnosis
dc.subjectFalse Positive Reactions
dc.subjectFemale
dc.subjectGestational Age
dc.subjectHumans
dc.subjectInfant, Newborn
dc.subjectLikelihood Functions
dc.subjectMale
dc.subjectPredictive Value of Tests
dc.subjectProspective Studies
dc.subjectSensitivity and Specificity
dc.subjectSepsis
dc.subjectTime Factors
dc.subject.classification7 INGENIERÍA Y TECNOLOGÍA
dc.titleDiagnostic accuracy of buffy coat culture compared to total blood culture in late-onset sepsis of the newborn
dc.typeArtículo
dc.identifier.volume17
dc.identifier.issue2
dc.identifier.startpagee110
dc.identifier.endpagee114
refterms.dateFOA2018-10-18T21:51:02Z


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