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Protective Effects of Moringa oleifera on HBV Genotypes C and H Transiently Transfected Huh7 Cells

dc.creatorJuan Socorro Armendariz Borunda
dc.date2017
dc.date.accessioned2018-10-18T20:34:59Z
dc.date.available2018-10-18T20:34:59Z
dc.identifier.issn23148861
dc.identifier.doi10.1155/2017/6063850
dc.identifier.urihttp://hdl.handle.net/11285/630409
dc.descriptionChronic hepatitis B infection treatment implicates a long-lasting treatment. M. oleifera extracts contain compounds with antiviral, antioxidant, and antifibrotic properties. In this study, the effect of M. oleifera was evaluated in Huh7 cells expressing either HBV genotypes C or H for the antiviral, antifibrotic, anti-inflammatory, and antioxidative responses. Huh7 cells were treated with an aqueous extract of M. oleifera (leaves) at doses of 0, 30, 45, or 60 μg/mL. The replicative virus and TGF-β1, CTGF, CAT, IFN-β1, and pgRNA expressions were measured by real time. HBsAg and IL-6 titers were determined by ELISA. CTGF, TGF-β1, IFN-β1, and pgRNA expressions decreased with M. oleifera treatment irrespective of the HBV genotype. HBsAg secretion in the supernatant of transfected Huh7 cells with both HBV genotypes was decreased regardless of the dose of M. oleifera. Similar effect was observed in proinflammatory cytokine IL-6, which had a tendency to decrease at 24 hours of treatment. Transfection with both HBV genotypes strongly decreased CAT expression, which is retrieved with M. oleifera treatment. M. oleifera treatment reduced fibrosis markers, IL-6, and HBsAg secretion in HBV genotypes C and H. However, at the level of replication, only HBV-DNA genotype C was slightly reduced with this treatment. © 2017 Sina Feustel et al.
dc.languageeng
dc.publisherHindawi Limited
dc.relationhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85042464640&doi=10.1155%2f2017%2f6063850&partnerID=40&md5=52c98157653f0a7472313ac796861d82
dc.relationInvestigadores
dc.relationEstudiantes
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.sourceJournal of Immunology Research
dc.subjectbeta1 interferon
dc.subjectcatalase
dc.subjectconnective tissue growth factor
dc.subjectgenomic RNA
dc.subjecthepatitis B surface antigen
dc.subjectinterleukin 6
dc.subjectMoringa oleifera extract
dc.subjectpregenomic RNA
dc.subjecttransforming growth factor beta1
dc.subjectunclassified drug
dc.subjectantiinflammatory activity
dc.subjectantioxidant activity
dc.subjectantiviral activity
dc.subjectArticle
dc.subjectcontrolled study
dc.subjectdrug screening
dc.subjectenzyme linked immunosorbent assay
dc.subjectgene expression
dc.subjectgenotype
dc.subjecthepatitis B
dc.subjectHepatitis B virus
dc.subjectHepatitis B virus genotype C
dc.subjectHepatitis B virus genotype H
dc.subjectHuh-7 cell line
dc.subjectliver protection
dc.subjectMoringa oleifera
dc.subjectnonhuman
dc.subjectplant leaf
dc.subjectprotein secretion
dc.subjectsupernatant
dc.subject.classification7 INGENIERÍA Y TECNOLOGÍA
dc.titleProtective Effects of Moringa oleifera on HBV Genotypes C and H Transiently Transfected Huh7 Cells
dc.typeArtículo
dc.identifier.volume2017
refterms.dateFOA2018-10-18T20:34:59Z


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