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Supercritical CO2 foaming of thermoplastic materials derived from maize: Proof-of- concept use in mammalian cell culture applications

dc.creatorErika García López
dc.creatorRoberto Portillo Lara
dc.creatorDiana Araiz Hernández
dc.creatorSilverio García Lara
dc.creatorMario Moisés Alvarez
dc.creatorCiro Angel Rodríguez González
dc.date2015
dc.date.accessioned2018-10-18T20:34:50Z
dc.date.available2018-10-18T20:34:50Z
dc.identifier.issn19326203
dc.identifier.doi10.1371/journal.pone.0122489
dc.identifier.urihttp://hdl.handle.net/11285/630372
dc.descriptionBackground Foams are high porosity and low density materials. In nature, they are a common architecture. Some of their relevant technological applications include heat and sound insulation, lightweight materials, and tissue engineering scaffolds. Foams derived from natural polymers are particularly attractive for tissue culture due to their biodegradability and bio-compatibility. Here, the foaming potential of an extensive list of materials was assayed, including slabs elaborated from whole flour, the starch component only, or the protein fraction only of maize seeds. Methodology/Principal Findings We used supercritical CO<inf>2</inf> to produce foams from thermoplasticized maize derived materials. Polyethylene-glycol, sorbitol/glycerol, or urea/formamide were used as plasticizers. We report expansion ratios, porosities, average pore sizes, pore morphologies, and pore size distributions for these materials. High porosity foams were obtained from zein thermoplasticized with polyethylene glycol, and from starch thermoplasticized with urea/formamide. Zein foams had a higher porosity than starch foams (88% and 85%, respectively) and a narrower and more evenly distributed pore size. Starch foams exhibited a wider span of pore sizes and a larger average pore size than zein (208.84 vs. 55.43 μm2, respectively). Proof-of-concept cell culture experiments confirmed that mouse fibroblasts (NIH 3T3) and two different prostate cancer cell lines (22RV1, DU145) attached to and proliferated on zein foams. Conclusions/Significance We conducted screening and proof-of-concept experiments on the fabrication of foams from cereal-based bioplastics. We propose that a key indicator of foamability is the strain at break of the materials to be foamed (as calculated from stress vs. strain rate curves). Zein foams exhibit attractive properties (average pore size, pore size distribution, and porosity) for cell culture applications; we were able to establish and sustain mammalian cell cultures on zein foams for extended time periods. © 2015 Trujillo-de Santiago et al.
dc.languageeng
dc.publisherPublic Library of Science
dc.relationhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84929493649&doi=10.1371%2fjournal.pone.0122489&partnerID=40&md5=53c405a90eda8b4f4840cc0b282ed608
dc.relationInvestigadores
dc.relationEstudiantes
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.sourcePLoS ONE
dc.subjectcarbon dioxide
dc.subjectformamide
dc.subjectglycerol
dc.subjectmacrogol
dc.subjectplastic
dc.subjectplasticizer
dc.subjectsorbitol
dc.subjectstarch
dc.subjectthermoplastic
dc.subjectunclassified drug
dc.subjecturea
dc.subjectvegetable protein
dc.subjectzein
dc.subjectbiomaterial
dc.subject22RV1 cell line
dc.subject3T3 cell line
dc.subjectanimal cell
dc.subjectArticle
dc.subjectassay
dc.subjectcell adhesion
dc.subjectcell culture
dc.subjectcell proliferation
dc.subjectDU145 cell line
dc.subjectfibroblast
dc.subjectflour
dc.subjectfoam
dc.subjectfoaming
dc.subjectmaize
dc.subjectmammal cell
dc.subjectmouse
dc.subjectnonhuman
dc.subjectplant seed
dc.subjectporosity
dc.subjectprostate cancer cell line
dc.subjectsupercritical carbon dioxide foaming
dc.subjectanimal
dc.subjectcell culture technique
dc.subjectcell line
dc.subjecthuman
dc.subjectmaterials testing
dc.subjecttemperature
dc.subjectMammalia
dc.subjectZea mays
dc.subjectAnimals
dc.subjectBiocompatible Materials
dc.subjectCarbon Dioxide
dc.subjectCell Culture Techniques
dc.subjectCell Line
dc.subjectHumans
dc.subjectMaterials Testing
dc.subjectMice
dc.subjectPorosity
dc.subjectTemperature
dc.subjectZea mays
dc.subjectZein
dc.subject.classification7 INGENIERÍA Y TECNOLOGÍA
dc.titleSupercritical CO2 foaming of thermoplastic materials derived from maize: Proof-of- concept use in mammalian cell culture applications
dc.typeArtículo
dc.identifier.volume10
dc.identifier.issue4
refterms.dateFOA2018-10-18T20:34:50Z


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