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A quantitative PCR approach for quantification of functional genes involved in the degradation of polycyclic aromatic hydrocarbons in contaminated soils

dc.creatorShahsavari E.
dc.creatorAburto-Medina A.
dc.creatorTaha M.
dc.creatorBall A.S.
dc.date2016
dc.date.accessioned2018-04-09T17:15:18Z
dc.date.available2018-04-09T17:15:18Z
dc.identifier.issn22150161
dc.identifier.doi10.1016/j.mex.2016.02.005
dc.identifier.urihttp://hdl.handle.net/11285/628074
dc.descriptionPolycyclic aromatic hydrocarbons (PAHs) are major pollutants globally and due to their carcinogenic and mutagenic properties their clean-up is paramount. Bioremediation or using PAH degrading microorganisms (mainly bacteria) to degrade the pollutants represents cheap, effective methods. These PAH degraders harbor functional genes which help microorganisms use PAHs as source of food and energy. Most probable number (MPN) and plate counting methods are widely used for counting PAHs degraders; however, as culture based methods only count a small fraction (<1%) of microorganisms capable of carrying out PAH degradation, the use of culture-independent methodologies is desirable.This protocol presents a robust, rapid and sensitive qPCR method for the quantification of the functional genes involved in the degradation of PAHs in soil samples.This protocol enables us to screen a vast number of PAH contaminated soil samples in few hours.This protocol provides valuable information about the natural attenuation potential of contaminated soil and can be used to monitor the bioremediation process. © 2016 The Authors. Published by Elsevier B.V.
dc.languageeng
dc.publisherElsevier B.V.
dc.relationhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84961190551&doi=10.1016%2fj.mex.2016.02.005&partnerID=40&md5=a1da21e5d9b77ac8f8d3f4368eccb3e0
dc.rightsopenAccess
dc.sourceMethodsX
dc.sourceScopus
dc.subjectpolycyclic aromatic hydrocarbon
dc.subjectArticle
dc.subjectbioremediation
dc.subjectDNA extraction
dc.subjectgene function
dc.subjectmicrobial community
dc.subjectmost probable number
dc.subjectnonhuman
dc.subjectoil spill
dc.subjectpolymerase chain reaction
dc.subjectquantitative polymerase chain reaction
dc.subjectreal time polymerase chain reaction
dc.subjectsoil degradation
dc.subjectsoil pollution
dc.titleA quantitative PCR approach for quantification of functional genes involved in the degradation of polycyclic aromatic hydrocarbons in contaminated soils
dc.typeArtículo
dc.identifier.volume3
dc.identifier.startpage205
dc.identifier.endpage211
refterms.dateFOA2018-04-09T17:15:18Z


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