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dc.contributor.authorZavala, Judith
dc.contributor.authorMontalvo-Parra, María-Dolores
dc.contributor.authorGuerrero-Ramírez, Guillermo-Isaac
dc.contributor.authorRodríguez-Barrientos, Carlos-Alberto
dc.contributor.authorTreviño, Victor
dc.contributor.authorValdez-García, Jorge E
dc.date.accessioned2018-02-07T17:28:40Z
dc.date.available2018-02-07T17:28:40Z
dc.date.issued2018-01-18
dc.identifier.citationBMC Research Notes. 2018 Jan 18;11(1):48
dc.identifier.urihttp://dx.doi.org/10.1186/s13104-018-3174-3
dc.identifier.urihttp://hdl.handle.net/11285/628003
dc.description.abstractAbstract Objectives Corneal endothelial cell (CEC) isolation and harvest aim to produce engineered grafts to solve donor corneal tissue shortage. To yield high amounts of CEC maintaining morphological and molecular characteristics, several isolation and culture conditions are reported. Here, we combined direct explant culture, with three different coating conditions and a two-step media approach to compare confluence efficiency, morphology, and specific molecular markers expression. Data description Confluence was reached after 2 weeks in the three coating conditions (Matrigel, collagen I, and in uncoated plates) using a two-step approach (proliferative medium without pituitary extract, followed by stabilizer basal medium). Na/K-ATPase and GPC4 markers were detected by immunocytochemistry while GPC4, CD200, and TJP1 by RT-PCR in the three CEC coating culture conditions. CEC in proliferative medium showed spindle morphology in the three conditions. Polygonal morphology was seen in CEC cultures using basal medium under uncoated and collagen I coated plates. CEC cultured in Matrigel-coated plates remained with spindle morphology in basal medium.
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titlePrimary explant culture and collagen I substrate enhances corneal endothelial cell morphology
dc.typeJournal Article
dc.language.rfc3066en
dc.rights.holderThe Author(s)
dc.date.updated2018-01-21T04:17:16Z
refterms.dateFOA2018-03-17T01:03:57Z
html.description.abstractAbstract Objectives Corneal endothelial cell (CEC) isolation and harvest aim to produce engineered grafts to solve donor corneal tissue shortage. To yield high amounts of CEC maintaining morphological and molecular characteristics, several isolation and culture conditions are reported. Here, we combined direct explant culture, with three different coating conditions and a two-step media approach to compare confluence efficiency, morphology, and specific molecular markers expression. Data description Confluence was reached after 2 weeks in the three coating conditions (Matrigel, collagen I, and in uncoated plates) using a two-step approach (proliferative medium without pituitary extract, followed by stabilizer basal medium). Na/K-ATPase and GPC4 markers were detected by immunocytochemistry while GPC4, CD200, and TJP1 by RT-PCR in the three CEC coating culture conditions. CEC in proliferative medium showed spindle morphology in the three conditions. Polygonal morphology was seen in CEC cultures using basal medium under uncoated and collagen I coated plates. CEC cultured in Matrigel-coated plates remained with spindle morphology in basal medium.


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