Analysis of the Dynamics of Neurosecretory Vesicles by Optical Tweezers and Image Processing
Alvarez Elizondo, Martha B.
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We present the analysis of chromaffin vesicles' dynamics during exocytosis. Optical tweezers combined with fluorescence for noninvasive microviscometry in cells and confocal imaging for trajectory analysis were used for this study. By the use of optical tweezers and fluorescent we applied the oscillation method propose by Fischer et al for measuring the intracellular vis- coelastic properties in cells. A sinusoidally moving optical trap was used to drive intracellular optical trapped vesicles present in chromaffin cells in order to obtain local information about the viscoelastic liquid surrounding the vesicles. For probing this technique measurement were performed on water, glycerol and PEO, then in cells.