Ciencias Exactas y Ciencias de la Salud
Permanent URI for this collectionhttps://hdl.handle.net/11285/551014
Pertenecen a esta colección Tesis y Trabajos de grado de los Doctorados correspondientes a las Escuelas de Ingeniería y Ciencias así como a Medicina y Ciencias de la Salud.
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- Development of a candidate bacteriophage M13-based vaccine against Rhipicephalus microplus (cattle tick)(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2021-06-09) González Mora, Alejandro; GONZALEZ MORA, ALEJANDRO; 708102; Benavides Lozano, Jorge Alejandro; emijzarate, emipsanchez; Carrión Chavarría, Belinda del Carmen; Martínez Torre, Ana Carolina; Hernández Pérez, Jesús; Escuela de Ingeniería y Ciencias; Campus Monterrey; Rito Palomares, Marco AntonioCattle tick (Rhipicephalus microplus) related diseases represent as a severe problem in cattle industry worldwide. This ectoparasite has caused huge economic losses in Mexico and around the world, which has been estimated in billions of dollars annually. Currently, the chemical acaricides represents the most widely used control method. However, several problems such as resistance to these drugs and contamination have been associated to this strategy. For such reasons, new approaches have been proposed to control tick infestations. Undoubtedly, the development of a vaccine is the most important of these strategies. Phage-based vaccines represent a fast and low-cost tool for antigen delivery. In this regard, the objective of the present work was to develop a candidate phage-based vaccine displaying a cattle tick antigen (Bm86-derived Sbm7462 antigen) on the surface of bacteriophage M13. Phage ELISA and dot blotting analysis confirmed the display of the antigen. A 2.73-fold increase in antigen expression was found compared to control (blank). Sbm7462 antigen was expressed in 0.5 µg of protein per 1 × 1011 PFU. Vaccine immunogenicity was evaluated using a bovine monocyte-derived dendritic cell-based ex vivo assay and a murine in vivo assay. The ex vivo model showed the maturation of dendritic cells after being pulsed with the phage-based vaccine (1.5-fold increase in CD80 and 1.6-fold in MHC-II when compared to unstimulated cells). The humoral response was confirmed in the in vivo assay. These results demonstrated the capacity of the phage-based vaccine to induce both humoral and cellular immune-specific responses. Importantly, this is the first report describing a control method for cattle ticks using a candidate phage-based vaccine. Further studies to evaluate the immunogenicity in a bovine model are needed. Thus, our candidate phage-based vaccine is a promising alternative for the control of cattle ticks.
- Effects of sound on growth, viability, protein production yield and gene expression in Escherichia coli.(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2018-12-14) Acuña González, Edgar; ACUÑA GONZALEZ, EDGAR; 626737; Benavides Lozano, Jorge Alejandro; Campus MonterreyThe effect of sound on biological systems is a subject that has been previously explored, mainly in relation to its use to increase agricultural production. However, the potential of this phenomenon has not been exploited properly because present studies
- Characterization of the skin secretions of Dryophytes arenicolor and identification of Arenin, a novel Kunitz-like polypeptide(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2018-12) Hernández Pérez, Jesús; HERNANDEZ PEREZ, JESUS; 633966; Benavides Lozano, Jorge Alejandro; Cardineau, Guy A.; Campus Monterrey; Campus Monterrey; Campus MonterreyZootherapy is the treatment of human ailments with remedies made from animals and their products. Despite its prevalence in the traditional medical practices worldwide, research on this phenomenon has often been neglected in comparison to medicinal plant